Journal of Nutritional Science and Vitaminology
Online ISSN : 1881-7742
Print ISSN : 0301-4800
ISSN-L : 0301-4800
Estimation of Xyloglucan in Vegetables by Enzyme-Linked Immunosorbent Assay
Yoshiaki SONEYumiko FUJIKAWA
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1993 Volume 39 Issue 6 Pages 597-606

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Abstract
We prepared the antibody specific against plant xyloglucan and applied it to enzyme-linked immunosorbent assay in order to estimate xyloglucan contents in several vegetables. In this experiment, xyloglucan was detected in the 24% aqueous potassium hydroxide-soluble fractions of tomato, cabbage, lettuce, and eggplant. The xyloglucan contents of these vegetables were estimated to be 1.4 mg, 54μg, 14μg, and 4μg per one hundred grams of their edible portions, respectively, using tamarind xyloglucan as a standard xyloglucan.
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