Effect of Feeding Clofibrate-Containing Diet on the Hepatic NAD⁺ Level in Rats

Abstract

Feeding rats with a diet containing 0.25% clofibrate for 2 weeks elevated the hepatic NAD⁺ and total nicotinate levels significantly. Other peroxisome proliferators, such as 2-(4-chlorophenoxy)propionic acid and di(2-ethylhexyl)phthalate, had similar effects. When rats were fed the control diet without clofibrate for 1 week after 2 weeks of the clofibrate diet, the hepatic NAD⁺ level returned to the control value. Muscular NAD⁺ content was not affected by the peroxisome pro-liferators. The results were discussed in relation to induction of peroxi-somal β-oxidation enzymes by the peroxisome proliferators.