Isolation of 9Z β-Carotene from Dunaliella bardawil and Its Stereoselective Synthesis

Abstract

An isolation of 9Z β-carotene from Dunaliella bardawil was accomplished by low-pressure column chromatography with Ca(OH)₂ column. Furthermore, it was stereoselectively synthesized by Wittig re-action between the C₁₀-phosphonium salt 2 and 9Z β-apo-8'-carotenal 1, which was prepared by Emmons-Horner reaction of 9Z β-ionylidene-acetaldehyde 3 with phosphonates 6 and 7 or 8a and 9.