Abstract
Objectives: In this study, we investigated the influence of the differences in exercise duration on alveolar and cytokines.
Methods: We randomly allocated 28 wild type mice into four groups: the exercise, non-exercise, four weeks (4W), and twelve weeks (12W). We induced the chronic obstructive pulmonary disease (COPD) in mice of all groups, when they were 12-14 weeks old, then disuse muscle atrophy was induced when they were 15-16 weeks old, to develop the COPD muscle atrophy model mice. Then, the exercises were performed until mice were 20 or 28 weeks old. The mRNA expression levels were analyzed by real-time PCR, the morphological analysis of the histological image was obtained from general staining, the macrophage expression level was examined using immunostaining, and pulmonary function was measured using spirometry.
Results: IL-13 in the lungs decreased in the exercise (4W) group, and IL-4 and TNF-α decreased in the exercise (12W) group. On the other hand, cathepsin-L increased in the non-exercise group (12W) (p ＜ 0.01-0.05). The differences in the exercise duration also affected TNF-α and cathepsin-L expressions. Mean alveolar diameter and the number of macrophages decreased in the exercise group (p ＜ 0.01-0.05).
Conclusions: Given this, it was elucidated that the difference in exercise duration affected the cytokines and macrophages, resulting in morphological changes.
