2025 Volume 55 Issue 1 Pages 23-28
Background: Basophils circulating in the blood play a proinflammatory role at sites of allergic inflammation in two stages: tissue accumulation and activation. This study analyzed the activation-enhancing effects of chemokines on chemokine-pretreated basophils using a two-step stimulation model.
Methods: Basophils were obtained from volunteers with no history of allergic disease. Cells were pretreated with chemokines for 30 minutes and then activated for 45 minutes with IgE-mediated stimulus plus chemokines. The percent histamine release of basophils was measured.
Results: The activation-enhancing effect of MCP-1 in the presence of basophils (24.3％±6.3％) was significantly decreased (6.8％±4.1％) when the cells were pretreated with MCP-1 (p ＜ 0.01). On the other hand, the enhancing effect of eotaxin (9.5％±8.0％) was maintained (8.8％±3.8％) when the cells were pretreated with eotaxin (p ＞ 0.05).
Conclusion: The results indicate that basophils retain their histamine release-enhancing effect when eotaxin is used at both the pretreatment and activation steps. However, the activation enhancing effect of MCP-1 was reduced when basophils were pretreated with MCP-1. Our two-step model of human basophil pretreatment and activation showed different patterns of chemokine action.
