2021 年 55 巻 2 号 p. 239-243
During osteoclastogenesis, various genes are expressed and functionally activated. Numerous studies have reported the important roles of various specific genes during osteoclastogenesis. However, it is still difficult to deliver genes into pre‐osteoclasts and mature osteoclasts; thus, virus transduction is generally used for overexpression studies in these cells. We investigated the effect of the KALA peptide, a low molecular weight cationic peptide, on lipid‐based transfection in the RAW264.7 (RAW) cell line. The addition of KALA peptide to conventional lipid‐based transfection reagents improved the transfection efficiency of cherry fluorescence protein that was evaluated by the number of fluorescence‐positive cells and mRNA expression level. We used RAW‐derived mature osteoclasts to investigate whether the addition of KALA peptide has beneficial effects on plasmid DNA delivery into mature osteoclasts. The lipid‐based transfection reagents with the KALA peptide enabled the expression of plasmid DNA in mature osteoclasts. Further, the lysosomal protein TPC2 was examined to confirm the beneficial effects of the KALA peptide. The delivery of TPC2 genes to cells was greatly improved by the addition of the KALA peptide. Our development of transfection methods for RAW cells and RAW‐derived mature osteoclasts could accelerate studies on the role of specific genes during osteoclastogenesis. (J Osaka Dent Univ 2021; 55: 239‐243)
