骨髄間質細胞の老化に関する研究
第1編 ヒト骨髄間質細胞の細胞構成比率,増殖動態及び造血因子産生能に及ぼす加齢の影響
1993 年 105 巻 5-6 号 p. 589-600
詳細
1993 年 105 巻 5-6 号 p. 589-600
Bone marrow stromal cells (BMSC) play essential roles in hemopoiesis. In the present study, bone marrow specimens obtained from 23 normal volunteers were cultured with a modified version of Dexter's long-term bone marrow culture system to investigate the influences of aging on human BMSC. The composition of BMSC was analyzed by immunofluorescence. The percentage of monocyte/macrophages, adipocytes and T-cells were significantly increased in the aged. BMSC from the aged proliferated more rapidly than those from the young during the first week of culture in the growing dynamics study. Cytokine concentration in the conditioned medium was also measured in terms of BMSC function by radioimmunoassay or enzyme-linked immunosorbent assay. The concentration of macrophage colony stimulating factor (M-CSF) on day 21 increased in the culture from the young, while interleukin-6 (IL-6) concentration was higher on day 7 in the culture from the aged. These findings show no evidence of BMSC defects in the aged. They show evidence suggesting advanced function.
