ヒト骨髄間質細胞に関する研究

第2編 慢性骨髄性白血病,骨髄異形成症候群,再生不良性貧血におけるヒト骨髄間質細胞の増殖動態並びに細胞構成の検討

抄録

Growth and composition of human bone marrow stromal cells (HBMSC) in CML, MDS and AA were studied by using modified Dexter's culture system. Growth was expressed by the percent of HBMSC at the bottom of the culture dish (% confluence) at the 1st, 2nd, 3rd and 4th week. Cells, harvested at the 4th week, were identified by monoclonal antibodies (anti-fibronectin, anti-vWF, anti-CD14, anti-CD7, anti-CD4 and anti-CD8 antibodies) and oil red 0 staining method.
In normal healthy individuals (NC), % confluence at the 1st, 2nd, 3rd and 4th week were 74±4%, 82±13%, 100% and 100% respectively (n=10). HBMSC were composed of 64±4% fibroblastoid cells, 15±3% endothelial cells, 9±4% monocyte/macrophage, 3±2% fat cells, 7±3% T lymphocyte, 4±1% helper/inducer T cells and 4±1% suppressor/cytotoxic T cells, respectively (n=8). In CML (n=6), % confluence at each week were almost on the decrease in comparison with those in NC and the cells tended to be detached from the bottom at the 3rd and 4th weeks. The composition of the HBMSC were not different in comparison with those in NC. In MDS (n=4), % confluence at each week and composition of HBMSC were not different in comparison with those in NC. In 3 of the 4 AA, % confluence at each week were not different in comparison with those in NC. Fat cells were markedly increased in one of 4 cases, which clinically belonged to the severe type of AA. In the other 3 AA, the percentage of endothelial cells was increased up to 35%, 32% and 21%, respectively. The CD4/CD8 ratios were not different from those in NC.
These findings indicate that the pathophysiological analysis of hematological disorders should be done not only on hematopoietic stem cells but also on the hematopoietic microenvironment including bone marrow stromal cells.