EXPERIMENTAL STUDIES ON JAPANESE B ENCEPHALITIS WITH THE RADIOACTIVE P³²

1. INFLUENCE OF P³² UPON THE NUCLEIC ACID QUANTITIES IN THE ORGANS OF MICE INFECTED INTRAPERITONEALLY WITH JAPANESE B ENCEPHALITIS VIRUS, WITH SPECIAL REFERENCE TO THE BRAIN, LIVER

Abstract

In this experiment were used the Mice injected intraperitoneally with 25μc of P³² twenty four hours after they were inoculated with Japanese B Encephalitis Virus.
And periodically they were quantitatively investigated. Seven days and ten days after the infection, certain quantities were collected out of each of these organs; and they were fractioned by Schmidt and Thannhauser's method into DNA-P and RNA-P; whose quantity was calculated by Fiske- Subbarow's method.
DNA-P and RNA-P in each organ of healthy mice were used as control objects for this experiment.
When healthy mice were injected intraperitoneally with the same amount of P³², RNA-P decreased only in liver; but, DNA-P remarkably decreased in each organ.
The result due to administration with 25μc of P³² to the mice which were inoculated intraperitoneally with Japanese B Encephalitis Virus previously, was similiar to that of healthy mice
RNA-P decreased a little only in liver, and DNA-P decreased remarkably in each organ. It was also found out that the time of death and of onset were both postponed.
It is widely known that DNA-P increased in each organ when mice are infected with the Virus. But in my investigation DNA-P decreased. So I cannot help concluding that β ray of P³² prevented the multiplication of Japanese B Encephalitis Virus.