家兎骨髓体外組織培養に関する研究
第1編 培地滲透壓の影響について
1956 年 68 巻 8special 号 p. 1-13
詳細
1956 年 68 巻 8special 号 p. 1-13
The influences of osmotic tension upon the tissue culture of rabbit's bone marrow were investigated.
The proliferation of cultivated tissue was expressed and examined numerically by the proliferation-index which was introduced by the density and the proliferation area.
The cell proliferation was accelerated in the hypotonic medium but not so markedly as described hitherto. In the hypertonic medium, it was suppressed.
When the serum plasma was used as the culture medium in various dilutions prepared with Locke's solution, maximal proliferation of the tissue was observed at one fourth of the initial serum concentration.
In the hypotonic medium, the migration of pseudo-eosinophilic leukocyte looked somewhat irritated at first but soon it became tardy. On the other hand, in the hypertonic medium, the migration was suppressed a little at first but it gradually recovered its initial activity.
These facts seem to show that the physico-chemical direct effects of pharmaca should not be over-looked when the effects of pharmaca upon the tissue culture of rabbit's bone marrow is studied.
