家兎骨髓体外組織培養に関する研究
第3編 培地水素イオン濃度の影響について
1956 年 68 巻 8special 号 p. 27-37
詳細
1956 年 68 巻 8special 号 p. 27-37
The influences of hydrogen ion concentration of media upon the tissue culture of rabbit's bone marrow were investigated.
On the course of culture the acidity of media gradually increased and in Carrel's bottle method pH-value changed from 7.80 to 7.06 in 6 days' culture.
When cultured in acid medium more prosperous proliferation was obtained with the use of comparatively lower acidity. Likewise, lower alkalinity proved more favorable for good proliferation.
In cases where good proliferations were obtained, the migration of pseudo-eosinophilic leukocyte was also vigorous.
Tyrode's solutions of various pH-values were prepared by adding adequate doses of sodium bicarbonate, and were used by mixing them with the media to find out the optimum pH-value for the tissue culture of rabbit's bone marrow. The maximal proliferation was obtained at pH 7.63 which was prepared by mixing Tyrode's solution of pH 8.00.
No significant changes in the migration rates of pseudo-eosinophilic leukocytes were caused by the added Tyrode's solution of various pH-values.
Thus the culture medium most favorable for the growth of tissue was decided as such of the hydrogen ion concentration of pH 7.63.
