Influences of Physiochemical Factors on the Motility of Pseudoeosinophils in Bone-Marrow Tissue Culture of Rabbits

Part 1. The Effect of Temperature on the Tissue Culture

Abstract

By observing the influences of temperature on the motility of pseudoesinophils in bone-marrow tissue culture of rabbits (cover-slip method) the author obtained the following results:
1. The limit of temperature that enables such observations lies in the range between 15°C, the minimum and 43°C, the maximum.
2. The maximum wandering velocity is found at 39°C, whereas the peak of the wandering velocity appears faster at a higher temperature and slower at a lower temperature.
3. As for the movement pattern, at the temperature showing the peak of wandering velocity, pseudoeosinophils of Types A and B appear most numerously; and also many pseudoeosinophils of Type F can be seen at a higher temperature.
4. At a certain stage in the limit temperature of both the maximum and minimum (43°C, 15°C) there appear a similar specific type, namely, the cell presenting a dumbbell-like shape having pseudopodia connected with the cell body at their veryr narrow part.
5. As for the shapes of pseudopodia, those presenting tongue-shape or saw-like ones tend to be most numerous at any temperature, and those with bleb shape or flag shape tend to be more numerous at a higher temperature. Those possessing no pseudopodia appear more at a lower temperature.
6. Relatively numerous tail-threads appear in an early stage at temperature of 37°C, 39°C, or 41°C, but generally these do not appear at a lower temperature, with one exceptionally peculiar thing is that only in an early stage at the low temperature of 15°C cells with many tail-threads make their appearance.
7. Either at the temperature lower than 30°C or higher than 39°C, and with the lapse of time the ratio of the long axis against the short axis grows smaller.