Abstract
The effect of supplementary substances to the basal media, Korthof's media, on the growth of Leptospira was investigated. The micro-organisms used in this experiment were L. hebdomadis, L. hebdomadis A, L. australis, L. icterohaemorrhagiae, L. pyrogenes and L. canicola, which were the departmental stock of authors laboratory.
And the following results were obtained.
1) It was found that rabbit serum to be added to Korthof's media could be replaced by bovine or equine serum.
2) The added serum exerted the activity as the essential element for growth of Leptospira in spite of heating at 60°C. for 30 minutes in twice.
3) The activity for growth of Leptospira of serum or Korthof's media containing serum was not spoiled by dehydration of them.
4) In the study of amino acid requirement of L. hebdomadis and L. icterohaemerrhagiae, it was found that aspartic acid was most preferable in the amino acids studied; and methionine was more suitable than cysteine for sulfur-containing amino acid.
