胃癌患者の赤血球に関する研究

第2編 放射性同位元素P³²による胃癌患者赤血球膜の透過性についての研究

抄録

By labelling erythrocytes of normal person with radio active isotope ³²p and adding the serum of gastric-cancer patient with the same type of blood to this, in vitro observations were carried on the gradual hemolytic phenomena, and thus the migration of ³²p to the serum was periodically determined. As the control similarly sera of gastroduodenal ulcers patient with the same type of blood was added to the labelled erythrocytes. By comparing the results of periodical determinations in the two groups with a view to discover difference, the following were obtained.
1) Cases in which serum of patient was added to erythrocytes labelled with ³²p: immediately after addition of serum cancer or ulcer patient almost no migration of ³²p to the serum can be observable in either case, but abcut, two hours afterward ³²p quickly migrate to the patient's serum. Thereafter ³²p invariably migrate more to the serum of patient, and by 24 hours hemolysis is completed and by that time the rate of migration of ³²p becomes equal and no more difference can be recognized.
2) Cases in which patient's serum and 10 mg/dl of hydrochloric hydroxylamine were added to the erythrocytes labelled with ³²p immediately after addition extremely little of ³²p is observed to migrate rapidly to the patient's serum. Thereafter ³²p migrates more to the serum of cancer patient, but it is characteristic that from the standpoint of time element ³²p migrates rapidly both to sera of cancer and ulcer patients. Eighteen hours later the ³²p migration becomes the same in the two.
3) Cases in which patient's serum and 50 mg/dl of hydrochloric hydroxylamine were loaded to the ³²p-labelled erythrocytes: immediately after addition a considerable amount of ³²p is already recognized to migrate to the serum. In this instance also more of ³²p migrates to the serum of cancer patient. Because the ³²p migration starts immediately after addition, there is no tendency to migrate rapidly from the standpoint of time element at first as abserved in the case where 10 mg/dl of hydrochloric hydroxylamine was added. About 16 hours later hemolysis is completed and the rate of migration becomes the same in the two cases. Even in this experiment from the macroscopic hemolytic phenomena, the completion of hemolysis is observable earlier in the serum of cancer patient.
4) Erythrocte resistance of the ³²p-labelled erythrocytes: By labelling erythrocytes of cancer and ulcerpatients each with ³²p, and by adding diluted physiological saline solution at various concentrations to each erythrocyte, one hour after addition supernatants are obtained from respective group by centrifugation and amount of ³²p in each supernatant is determined. The rate of migration of ³²p to physiological saline solution shows a very little difference in the concentrations of 0.6% and 0.5% solutions, but with concentrations of 0.35% and 0.3% saline solutions in cancer patient a greater amount of ³²p migrate than in the case of the control.
5) Cases in which patient's serum after resection of the stomach for gastric cancer was added to erythrocytes labelled with ³²p: In addition of the patient's serum which coursed 2 weeks after resection of the stomach, migration of ³²p to the serum of cancer patient decreased and the rate of migration of ³²p becomes no mofe difference can be recognized between serum of cancer and ulcer patients.
Cases iu cancer patient who underwent merely exploratory laparotomy because radical operation was impossible to be performed, migration of ³²p to the serum becomes more clearly in comparison with ulcer patient.