Abstract
The author tried purification of Rickettsia tsutsugamushi, and electron microscopic observation of the purified rickettsiae, and also studied electron microscopic figures of the ultrathin sections of the mouse organs and tissures infected with Rickettsia tsutsugamushi. The results are summarized as follow:
1) Both of the rickettsiae purified and observed in the ultra-thin sections of the abdominal exsudate cells were of various figures. The size of purified rickettsiae is 0.6 to 1.0 μ, and that of those in abdominal cells is 0.3 to 0.5 μ on the average.
2) A capsul-like substance could be observed around the purified rickettsiae, but the presence of such substance could not be proved around the ones in abdminal cells.
3) In more than a half of the rod-shaped purified rickettsiae, the so-called polarstaining-like structure could be observed of those in abdminal cells, some has the polarstaining like structure, but most of them were of no or somewhat granular structure.
4) In ultra-thin sections of abdminal cells, the presence of rickettsiae could be clearly obsered. In the liver and spleen particularly in the liver, the rickettsialike structures could be also observed, but the identification needs further studies,
