Abstract
With the use of the standard strains, namely, Staph. aureus, Staph. albus, Sh. flexneri 2a, Sal. typhi 57 S, and Sal. typhi 57 R stocked in our department, the author studied the relations between the quantities of nucleic acids, RNA and DNA, in the cells and the duration of cultures, and carbon sources in the media; and also investigated the quatitative changes of nucleic acids of the resting cells after shaking of bacterial suspension. The following results were obtained.
1) The quantity of RNA was found to be more laage in the cells of younger culture. It can be asid that the amount of RNA taking the part in protein synthesis was increased in the cells that art multiplying actively.
2) It was found rather large quantity of RNA in the case of bacteria, when it was cultured on the media that were containing carbon sources, especially glucose.
3) The quantity of RNA was gradually decreased by the shaking of the suspension of resting cells. However, it was observed the marked increase of the quantity, if glucose was added into the suspension. But the addition of peptone itself or combination with carbon source, like glucose, revealed decrease of the quantity.
