Experimental Studies on the Professional Allergic Skin Reactions induced by POCI₃

Part 3. Studies on the Sensitization of Animals with POCI₃

Abstract

The author studied the sensitization of animals with POCI₃. Namely guinea pigs and rabbits percutaneously sensitized to POCI₃ showed the allergic skin reaction by reinjecting POCI₃. And the results were as follows.
1) When POCI₃ was injected into the skin of normal guinea pigs, the direct skin reaction was kept positive up to 1/500 of POCI₃ in 0.1cc olive oil, after 24 hours.
2) When POCI₃ was injected into the skin of normal rabbits, the direct skin reaction was kept positive up to 1/1, 000 of POCI₃ in 0.2cc olive oil, after 24hours.
3) When 1/5, 0001/10, 000 of POCI₃ in 0.1cc olive oil was reinjected into the skin of intracutaneously sensitized guinea pigs, it showed the allergic skin reaction.
4) When 1/5, 0001/10, 000 of POCI₃ in 0.2cc olive oil was reinjected into the skin of rabbits actively sensitized by skin inunction, it showed the allergic skin reaction.
5) When the same quantity of POCI₃ was injected into the skin of normal animals and sensitized animals the skin reaction of sensitized animals was far more strong than that of normal animals.
6) From the above results it became clear that POCI₃ was a substance possible to sensitize animals.