Abstract
The reaction procese of hemoglobin in erythrocyte was spectrochemically observed on the upper clear solution after the hemolysis of separated erythrocyte with distilled water on the addition of 1-ascorbic acid to the floating solution of erythrocyte in physiological salt solution. And the results were as follows.
1. The formation of methemoglobin was only observed on the scanty dosis of 1-ascorbic acid to the number of erythrocyte, but the formation of the product with the absorption maximum at 630 mμ was merely observed after letting it alone for 40 hours.
2. The formation of methemoglobin and then the formation of the product with the absorption maximum at 670 mμ in erythrocyte were observed on the great dosis of 1-ascorbic acid to the number of erythocyte. At that time, this reaction was settled by the rats of the erythrocyte number (hemoglobin dosis) and the 1-ascorbic dosis. Methemoglobin was only produced in the acidic side of reaction solution and the formation of the product with the absorption maximum at 670 mμ was promoted in the alkaline side of reaction solution.
3. The formation of the product with the absorption maximum at 630 mμ and then the formation of the product with the absorption maximum at 670 mμ was promoted on the shaking process of reaction.
4. Methemoglobin was only produced on the aeration of oxygen to reaction solution or the aeration of oxygen to reaction solution with the shaking process.
5. Therefore, it was determined if hemoglobin in erythrocyte was decomposed to only methemoglobin or to the product with the absorption maximum at 670 mμ or to the product with the absorption maximum at 630 mμ by the permeability of 1-ascorbic acid to the erythrocyte membrane and the intensity of the influence of active oxygen followed by the selfoxydation of 1-ascorbic acid in the outside of erythrocyte.
