Abstract
For the purpose to study physico-chemical structures of surface area of the bacterial cell, the author has constructed various kinds of dielectric spectrometer and performed several fundamental experiments which permitted their biological applications, theoretically and technically.
Dielectric spectrometers have been designed and constructed so as to have sufficient output to measure the dielectric ratio of biologic systems through whole range from 100 killocycles to 3, 000 megacycles. Thus, dielectric spectrometers in our hands had three major apparatus: Heterodyne type spectrometer from 100 killocycles to 60 megacycles, Lecher's wire spectrometer from 60 megacycles to 600 megacycles, ultra-short wave spectrometer with wave guide from 100 megacycles to 3, 000 megacycles. By the way, these spectrometers have been never made in application of bilogical field until to date.
Utilizing these spectrometers, several important fundamental investigations have been performed and results were summarized as followed.
No marked evidence was found in its accuracy between King's method and Drude-Mizushima's method with Lecher's wire spectrometer. Using Gelatine-gel system as a model of biologic material, minimal and sufficient size of receptacle has been determined in details. For examples, it was 10×15mm at wave length of 70cm. This result indicated the technical possibility to measure them in bacterial cell. In addition to Gelatin-gel, several mixed solutions, water-aceton, water-ethylacohol, water-metylalcohol etc were also spectrometerized. Effect of electrolytes on these materials were also determined and were identical with theoretical calibration by previously reported equations.
