Abstract
In the first item of this report, coordination of the surface substance with activity of adaptation enzyme has been studied. Purified polysaccharide-polypeptide-lipid compound had strong activity to promote the activity of adaptation enzyme, although ribonucleic acid-Mgpolysaccharide-polypeptide-lipid compound did not show any action on enzyme. Furthermore, this activity was strongest in polysaccharide-polypeptide compound. However, polypeptide or lipid had no such activity. Consequently, it was concluded that only the condition in which polysaccharide molecules were vailed on the surface of bacterial cell against both of intracellular and extracellular circumstances made it to promote the enzyme action.
In the second item, the author described the bacterial toxin obtained by shaking and its relation to surface structures of the bacterial cell. By shaking bacterial cell with ribonucleic acid purified from the same kind of bacteria, there were obtained two kinds of hemolytic toxin, e.g. one contained ribonucleic acid and other contained polysaccharide. When energy substance, such as glucose, was added sufficiently, latter was predominantly produced. On the contorary, when the mixture was shaked without energy substance, former was predominantly produced. Thus, intimate relation with energy metabolism was found in the production of this kind of toxin.
By shaking bacterial cell with polysaccharide-polypeptide-lipid compound, there obtained another kind of toxin which showed no intimate relation with energy metabolism.
