Antitumor Activity of the Regional Lymph Node Protein of Mice Transplanted with Ehrlich Ascites Tumor

Part 1. Fractionation on Sephadex G-200 Column Chromatography

Abstract

The regional lymph nodes of mice transplanted with Ehrlich ascites tumor have an inhibitory effect for cultured Ehrlich ascites tumor. The supernatant of homogenized regional lymph nodes were fractionated on Sephadex G-200 column chromatography. The elution curve was fractionated in main three peaks, and the later half of the first peak and second peak were effective for the suppression of Ehrlioh ascites cell growth. These two fractions were agreed electrophoretically protein.