分光々度法による人血液カタラーゼ活性の定量および低カタラーゼ血液症のスクリーニング法について

第1編 直読式分光々電光度計による正常血カタラーゼ活性の測定

抄録

Study on the frequency of hypocatalasemia, heterozygote of acatalasemia, has an extremely important bearing on acatalasemic gene distribution.
For the identification of hypocatalasemia or for its screening, it is essential to study the methods of quantitative analysis of blood catalase.
For such a quantitative analysis as well as for the screening test, a modified form of Euler-Josephson's method has been used by Okayama University Medical School and by Hiroshima and Nagasaki ABCC groups (Atomic Bomb Casuality Commission). This method is based on titration with potassium permanganate (KMnO₄) to determine decrease of hydrogen peroxide (H₂O₂) by blood catalase.
On the other hand, Beers et al. described a method of liver catalase assay for measuring the breakdown of hydrogen peroxide by estimating the decrease in the optical density at the wave length of 240mμ. As it is possible to check directly the decrease of H₂O₂ with this method, it seems to be the most simple and highly reliable method with little error.
The purpose of this paper is an attempt to determine the catalase activity of human blood and to apply the identification of hypocatalasemia and normal individuals and, if possible, to employ as an assay method for investigating hypocatalasemic blood catalase from the aspect of enzyme kinetics by spectrophotometry (ultraviolet method).
In this part an attempt was made to determine the catalase values of some blood samples. The results of this determination were compared with those obtained by the titration (modified Euler-Josephson's method) with the same samples under approximately the same condition.
It was observed that the catalase values as estimated by the ultraviolet method (UV method) were about 70 to 80 per cent lower than those by the titration.