日本脳炎ウイルスの電子顕微鏡的研究
1971 年 83 巻 1-2supplement 号 p. 17-24
詳細
1971 年 83 巻 1-2supplement 号 p. 17-24
Plaques produced by Japanese encephalitis virus (JEV) on the monolayer of chick embryo fibroblasts, formalin-treated purified JEV, supernatant of the culture media of JEV-inoculated chick embryo fibroblasts and JEV-inoculated mouse brain were studied electron microscopically to determine the size, shape and developmental process of JEV.
JEV particles observed were spherical, approximaterly 40mμ in diameter and coated with an envelope having spikes.
In plaques, numerous JEV particles formed aggregates, some of them displaying a crystalline arrangement, among degenerated mass of chick embryo fibroblasts. Some of the aggregates were observed to be wrapped with a membraneous structure in the cell debris.
Besides JEV particles, numerous virus particles presumably pertaining to avian sarcomaleukosis complex were seen among normal chick embryo fibroblasts.
In inoculated mouse brain, JEV particles were observed in the cytoplasmic vacuoles and intercellular spaces.
The finding above confirmed author's previous electron microscopic observation on JEV particles.
