腸管吻合創治癒過程の組織化学的,電子顕微鏡的研究

抄録

The healing process of anastomosed jejunum of rats is studied using radioautography and electron microscopy.
From three hours to seven days after operation, DNA synthesizing zone increases in the crypt cells around the anastomosed area. This phenomenon is not observed at later stages after operation.
Regenerative epithelial cells start to appear from the 2nd post-operative day. They are cubic in shape and their micro-villi are shorter and less numerous than those seen in the matured epithelial cells. In the regenerative epithelial cells, one seldom sees endoplasmic reticulum in contrast to numerous free ribosomes. Parallel to the post-operative days, villous regeneration has begun to form themselves like budding and consequently regenerative cells become more differentiated, resembling the matured villous cells.
In stroma around the anastomosed area, the numbers of fibroblasts rapidly increase at the 3rd post-operative day, and one observes extensive development and dilatation of endoplasmic reticula.
Small numbers of collagen fibrils are found extracellularly at the 4th post-operative day, and numbers of fibrils and their widths have continued to increase as the time lapses.
In malnutrition, DNA synthesizing zone in the crypt cells is on the lesser increase and the proliferation of connective tissue cells is in retard as compared with control animals.
Fibroblasts show autolytic degeneration at the 7th post-operative day in the ill-nourished animals.