抄録
A curude cornin extracted from rat liver microsomes and the chemical properties of the purified cornin (MF1) were investigated.
1. Crude rat liver microsomal cornin (RLMC) inhibits the growth of L-929 cells. MF1 is purified about 4 fold from RLMC by gel filtration through Sephadex G-50 column.
2. The concentration of MF1 that inhibits the growth of L cells to 50% is 270μg/ml and this specific activity is the highest of the cornin fractions reported up to date.
3. The content of acidic amino acids is more than 23% but that of histidine and sulfurcontaining amino acids is very low in MF1.
4. MF1 shows the maximum ultraviolet absorption at 276nm and its main substance is a kind of protein, and its inhibitory effect on the cell growth diminishes by protease treatment.
5. MF1 is a single peak both by polyacrylamide gel electrophoresis and by thin layer gel filtration and the molecular weight is calculated about 26, 000.
6. MF1 is separated in two bands by SDS-polyacrilamide gel electrophoresis and the molecular weight is estimated to be 11, 000 and 15, 000 respectively.
