Ultrastructural localization of carbonic anhydrase activity in developing enamel and dentin of the rat incisor
1983 年 25 巻 4 号 p. 1129-1133
詳細
1983 年 25 巻 4 号 p. 1129-1133
Localization of carbonic anhydrase (CA) in the developing dentin and enamel of the rat lower incisor was investigated by means of a histochemical technique at the electron microscope level. The CA reaction products were always localized in the area where initial mineralization i.e., nucleation of crystals occur in the developing dentin as well as enamel. In the enamel, CA activity was observed only in the initial marginal zone (10-20 μm in thickness) of the enamel at the matrix formation stage. The precipitates produced by the CA reaction appear in close proximity to the newly formed ribbon-shaped crystallites. CA activity in the dentin occurs in the calcification front (dentin-predentin border) of a few μm in thickness. Furthermore, the precipitates which appear on the predentin side are enclosed in the mass of initial crystals.
The present study confirms our previous observations at the light microscope level.
