1998 Volume 40 Issue 4 Pages 241-248
In forensic practice, the sex determination and the ABO blood group genotyping are the most fundamental methodused for personal identification.
PCR analysis offers the advantages of greater speed and sensitivity. We previously reported the forensic value of teeth as a source of DNA for genetic analysis and demonstrated conclusively that the Chelex-based filtration procedure utilizes DNA obtained from a whole tooth section suitable for PCR amplification.
Gender is determined by amplifying a segment of the X-Y homologous gene amelogenin. ABO genotype can be analyzed by PCR of genomic DNAs and digestion of the PCR products with restriction enzymes at different points of nucleotides can be determined.
Tooth samples from 25 females and 15 males extracted from 40 patients living in Kanagawa prefecture were gender typed using this test. Regarding ABO genotype, results showed, 10AA and 9AO in phenotype A, 2BB and 8BO in phenotype B. There was conflict observed in individuals with phenotypes AB and O. These results suggest that sex determination and ABO blood group genotyping methods in genomic DNA extracted teeth using PCR which are rapid, sensitive and reliable methods should be useful in forensic practice.
