Quantitative Analysis of Fatty Acid Composition by Paper Chromatography using Hydrogenation on Papers

Abstract

For the quantitative analysis of fatty acid composition of fatty oils, the application of paper-chromatography using hydrogenation on papers was examined, in addition to the usual reversed phase paperchromatography consisted of hydrocarbon as the stationary phase and acetonitril acetic acid as the mobil solvent. Complete hydrogenation on papers was achieved after 2 hr at 30, using palladium black as catalyst. By applying this analytical method, the composition of fatty acids of wheat germ oil and of skipper oil was determined as follows.
Wheat germ oil : C₁₆F₀ : 12.3%, C₁₈F₀ : 3.7%, C₁₈F₁ : 20.6%, C₁₈F₂ : 45.6%, C₁₈F₃ : 12.3%, C₂₀F₀ : 5.4%.
Skipper oil : C₁₄F₀ : 8.6%, C₁₆F₀ : 14.6%, C₁₆F₁ : 3.8%, C₁₈F₀ : 1.6%, C₁₈F₁ : 8.7%, C₁₈F₂, : 6.8%. C₁₈F₃ : 1.2%, C₂₀F₁ : 15.7%, C₂₀F_{4, 5} : 7.1%, C₂₂F₁ : 17.2% C₂₂F_{5, 6} : 14.7%