Abstract
Cardiolipin was prepared from the frozen heart muscle of fin whale according to Pangborn's method. Every step in the course purification was pursued by T.L.C., and the sample after the last step showed only single spot corresponding to cardiolipin on T.L.C.
From chemical analysis it's characteristics were as follows : P (%) -4.2%, N (%) -<O.1, I.V.-107.
There was no difference between the pattern of IR apectrum of ox cardiolipin and that of fin whale cardiolipin.
The composition of fatty acid calculated from the pattern of G.L.C. was as follows : Palmitic acid-23.5%, palmitoleic acid-4.5%, Oleic acid-44.5%, Linoleic acid-25.0%, Linolenic acid-1.4%, but the observed IV value did not coincide with that calculated from the composition of fatty acid based on G.L.C.
In comparison with ox cardiolipin, cardiolipin of fin whale was found adaptable to the antigen for the routin test for syphilis.
