Abstract
The extracts from soiled undershirts were obtained by ethylether and benzene ethanol (80/20) extraction.
The fiber surfaces of untreated and after extraction with solvents were observed by scanning electron microscope.
After qualitative analysis of two extracts carried out by IR spectrometer and thin-layer chromatography, the extract obtained with ethylether extraction was quantitatively separated into several lipid groups by column chromatography using dry column and silicic acid treated with isopropanol-KOH, and gas chromatography. The extract with benzene-ethanol was separated into urea, deteriorated materials and other organic compounds.
The composition of extracts consists of hydrocarbons (1.8%), squalene (9.3%), cholesterolesters and other sterolesters (2.0%), waxes (18.0%), triglycerides (20.1%), diglycerides (2.0%), monoglycerides (2.4%), free fatty acids (26.4%), free fatty alcohols (0.8%), cholesterol and other sterols (1.3%), urea (6.1%), deteriorated and unidetified materials (9.5%) and which is similar to those of Europian and American skin lipids.
