Abstract
The seeds of Ilex integra were extracted with petroleum ether. The fatty acid mixture obtained on saponification of the crude fats was esterified with methanol and concentrated sulfuric acid and then analyzed by GLC. The main component was octadecadienoic acid (linoleic acid).
As minor components, the presence of eicosanoic, and octadecadienoic acids was confirmed by using the GC-MS method and ECL-value. Moreover, by the same means, the presence of some odd numer acids, such as heptadecanoic, and nonadecadienoic acids-which are seldom found in natural fatty acids-were identified.
Authors believe that the combined use of the GC-MS method and ECL-value is an effective procedure for the determination of small amounts of fatty acids in natural compounds.
