Abstract
The complex (HA) of sodium α-olefin sulfonate (AOS) and human serum alubmin (HSA) were prepared by mixing each component dissolved in water (AOS/HSA-1/2), dialysing the mixture over night against water and centrifuging off the precipitant. HSA used was previously purified with ethanol (Korner's method) and then with active carbon in acidic medium. The formation of HA was confirmed qualitatively by the measurements of mobility and color reaction.
The complex containing 30mg of total protein was injected in rabbit by both subcutaneously and intravenously during the period of 21/2 months. According to thn ordinary precipititin reaction, authors found only 0 or 2' positive reactions in HA-anti HA system against 3' or 3 positive reactions in HSA-anti HSA system, and no reactions in AOS-anti HA and AOS-anti AOS systems respectively.
