Abstract
A method for the quantitative determination of surfactants containing NH groups in molecules was examined by thin layer chromatography. Surfactants containing NH groups such as long chain N-acylglutamates were chromatographed by a silicagel-coated plate and could be easily detected by N-chlorination and subsequent spraying with starch-potassium iodide solution. A minimum amount for detection was about 0.5μg. The color intensity was proportional to the concentration of a sample spotted, and therefore, the amount of the surfactant could be determined by comparison with the standard solution on the color intensity.
