Thin-Layer Chromatographic Determination of Neutral Lipids by Photodensitometer

Abstract

Responses of cholesterol oleate, cholesterol, oleic acid, tri-, di-, and monoolein to the internal standard (methyl oleate) on the TLC plates which were charred after spraying 10% sulfuric acid were determined using a photodensitometer of a zig-zag scanning mode. The relations between the ratio of peak area and that of weight were linear and reproducible over a range of loads (0750μg triolein and 0125μg other lipids) under the same conditions. The responses of cholesterol and its ester, however, were found to be variable by changing charring conditions. The quantitative analysis of yeast neutral lipid revealed that the method was useful and convenient for the estimation of neutral lipid composition indicating approximately 18% of maxium coefficient of variation.