Abstract
A method is described for the determination of ferulate in rice bran oils by high performance liquid chromatography using a detector set at 315 nm which is an absorption maximum of ferulate.
Previously reported wave length of the detector was 254 nm. The sensitivity of response for cholesteryl ferulate at 315 nm was 1.7 times higher than the sensitivity for it at 254 nm.
Operating conditions were Zipax-BOP column and the mixture of hexane and diethyl ether (85 : 15 vol/vol) as a mobile phase. Cholesteryl ferulate was used as an external standard.
Though components of ferulate were not still separated from each other, newly presented method is useful for the determination of the ferulate contents in refined rice bran oils.
