Abstract
The influence of cultural conditions, especially C/N ratio of medium, growth temperature and nitrogen source, on the amounts of lipids formed in the mycelium of three species of Pellicularia genera was investigated. It was found that the decrease of C/N ratio and the increase of growth temperature generally resulted in the increase of the amounts of fungal lipids. Maximum accumulation of lipids in the mycelium, 197 mg/200 ml medium, from glucose by using NH4NO3 as a nitrogen source occurred at a C/N ratio of 5.7 at 35°C by P, filamentosa sp. sasakii, IFO No. 8985. The fatty acid compositions of neutral and polar lipid fractions fractionated from lipid extracts by silicic acid column chromatography and the lipid compositions of both fractions were determined. The influence of the cultural conditions on these compositions was also investigated. High amounts of linoleic acid were present in both lipid fractions, particularly incubated at a C/N ratio of 5.7 at 20°C.
