The relation between area percentage and DEGS concentration

Studies on the Standardization of a GC Method for Content Determination of IPA, DHA in PUF-Fractionated Fish Oil. I

Abstract

For standardization of a quantitative analysis method of IPA (Icosapentaenoic acid) and DHA (Docosahexaenoic acid) in fish oil containing concentrated polyunsaturated fatty acid, the methyl esters of IPA at a content of about 25% and 18% were analyzed by the gaschromatography method of DEGS (Diethylene Glycol Succinate) packed column.
The experimental data were treated statistically to examine inter-and intraloboratory scattering. The inter-and intralaboratory CV (Coefficirnt of Variation) of DHA mean values appreciably increased more than those of the IPA mean values obtained at each laboratory. The CV of IPA mean values at 10% DEGS were less than 2.0% in either sample.