Abstract
From Bacillus subtilis ATCC No. 21813, barley β-glucanase and α-amylase were produced simultaneously in a submerged culture.
To obtain barley β-glucanase with less α-amylase the culture process using this Bacillus sp. was investigated.
The addition of (NH4)2HPO4 or FeSO4 to the broth filtrate depressed α-amylase activity while maintaining that of β-glucanase.
The addition of both these compounds to the culture medium gave the same results in the culture process. Essentially the same results were obtained using other Bacillus sp.
