Abstract
To standardize the assay method of lipase with the emulsified substrate, some important factors affecting lipase activity were investigated.
The coefficient of variation (C.V.) in lipase activity by lot of olive oil were different among origins of lipase. C.V. were less than 10% in the case of assaying 10 times in one assay (within-run) and its value in duplicate in twice at regular intervals (between-run) was approximately 12%.
The suitable agitation number to get the highest lipase activity varied with origins of lipase. The agitation number to be suitable for any origin of lipase was 15, 000 rpm to 16, 000 rpm.
To keep emulsified substrate stable, addition of the mixture of PVA 117 and PVA 205 was better than when either is added alone.
