Abstract
While increasing lipase production by Mucor javanicus IAM 6108, reduction in cultivation time and stabilization of lipase in the broth were investigated. The addition of 1 % soybean oil to the initial medium containing 3 % corn steep liquor, 1 % soluble starch, 0.2 % KH2PO4, 0.05 % MgSO4·7 H2O, 0.05 % KCl, and 1 % soybeanyuto reduced the cultivation time of lipase production from 50 h to 30h.
Lipase activity in the broth decreased rapidly after reaching a maximum. To avoid this, the 1 % soybean oil was added to the broth at 25 h of cultivation before the maximum had been reached, As a result, maximum lipase activity was maintained for more than 10 h. This appeared due to the presence of fatty acids or triglycerides with double bonds on the broth.
