Abstract
A simple and reliable method to qualify and quantify autoxidized triglycerides simultaneously was developed using reversed phase HPLC equipped with an RI detector.
When certain triglycerides such as olive, safflower and linseed oil were subjected to HPLC analysis, only one peak for unoxidized and four peaks for autoxidized triglycerides appeared on the chromatogram under the conditions used with hexane :methanol : 2-propanol (98:1:1) as the eluent. The four peaks of each autoxidized triglyceride, on the chromatogram were assigned as unoxidized triglycerides, di-, mono- and tri-hydroperoxides of triglycerides, respectively.
For the POV determination, the calibration curve was made from the linear relationship between the ratio of total peak area of hydroperoxides to that of all peaks on the chromatogram and POV of the sample triglyceride in the range of 301000 meq/kg. The POV thus determined was quite close to that measured by potentiometric titration.
