Determination of Lysophosphatidylcholine and Phosphatidylcholine in Partially Hydrolyzed Phospholipid by TLC-Enzyme Method

Abstract

We describe a new method for determination of phosphatidylcholine and lysophosphatidylcholine in partial hydrolyzed phospholipids. The method consists of fractionation of cholinecontaining phospholipid by preparative TLC and enzymatic determination based on the cholineoxidase-phenol method. The method affords better specificity and shorter time than the conventional two-dimensional TLC method are possible. Reproducibility (CV<3 %) and good agreement with those of two-dimensional TLC method were confirmed. This method is thus applicable to the determination of lysophosphatidylcholine and phosphatidylcholine in partially hydrolyzed phospholipids.