Abstract
A method was developed for determining of steryl glycosides (SG) in commercially available concentrated phospholipids. ASG (acylated SG) and NASG (non-acylated SG) were separated by TLC and determined by HPLC equipped with an evaporative light scattering detector (ELSD). Two separation modes [gel permeation (GP) and reversed phase (RP)] were examined in this study. A good linear correlation was obtained between the logarithm of concentration and peak intensity in ELSD. Individual calibration curves were essential for the determining of NAGS and ASG due to the dependency of ELSD on molecular weight. To simplify the method, ASG was determined following hydrolysis with 0.25 N KOH in methanol. Data for steryl and acyl group in ASG were obtained from acetylated ASG using the RP mode for separation. The limit of detection was 0.05 % for commercial concentrated phospholipids.
