Quantitative Analysis of Triacylglycerol by HPLC with a Light Scattering Detector

Abstract

A reversed phase high performance liquid chromatography (HPLC) system with a light scattering detector (mass detector) was established to determine the relationship between signal response and molecular species of triacylglycerol. Following the injection of sufficient amounts of samples, the detector showed very good reproducibility. Signal intensity from the detector increased with sample retention time. The logarism of sample amount was proportional to the logarithm of responsibility. Responsibility was influenced by nebulizing pressure and evaporator temperature of the detector. Quantitative analysis using a mass detector with a calibration curve gave results more accurate than those obtainable by a reflactive index detector.