Abstract
Neutral glycosphingolipids of the arthro-series were isolated from the porcine roundworm, Ascaris suum, Class Nematoda, Phylum Aschelminthes, Oligosaccharide structures of the glycolipids were completely characterized as Glc β1-Cer (CMS1 and CMS2), Man β1-4 Glc β1-Cer (CDS), GlcNAc β1-3 Man β1-4 Glc β1-Cer (CTS), GalNAc β1-4 GlcNAc β1-3 Man β1-4 Glc β1-Cer (CQS) and Gal α 1-3 GalNAc β1-4 GlcNAc β1-3 Man β1-4 Glc β1-Cer (CPS) by compositional analysis, methylation analysis, exoglycosidase hydrolysis, acid hydrolysis, chromium trioxide oxidation and TLC-immunostaining.
The fatty acid and sphingoid compositions of CMS1, CDS, CTS, CQS and CPS were virtually the same, with those of 2-hydroxy C24 : 0-acid and branched heptadecasphingosine and heptadeca-4-sphingenine predominating. There would thus appear to be a comman metabolic pathway for CMS1 to CPS. However, CMS2 differed completely from the sphingoid components of other glycolipids with trihydroxy bases. Thin-layer chromatography and the results of sugar analysis indicated the presence of at least four minor glycolipids possessing large oligosaccharide chains each with six, seven sugars.
