Abstract
High performance liquid chromatographic separation of two positional triacylglycerol isomers of 1, 3-dipalmitoy1-2-oleoylglycerol (POP) and 1, 2-dipalmitoyl-3-oleoyl-rac-glycerol (PPO) as bromine or ICl adducts was carried out on a reversed stationary phase column packed with 3 μm particles of LiChrospher 100CH-18. Good separation of the triacylglycerol positional isomers required about 130 min as the bromine adduct using acetone/acetonitrile (8 : 2 vol/vol) as the mobile phase. Palm oil was analyzed by this method under similar conditions and the proportion of PPO to POP in palm oil was determined.
