Abstract
The specificity of phospholipase D prepared from fresh cabbege leaves toward alcohols wasevaluated by transphosphatidylation between phosphatidylcholine (PC) and 15 different alcoholswith double bond or cyclic structures.
Based on the conversion ratios of PC to the corresponding phosphatidylalcohols at less than0.04 M alcohol in the water layer, whose activity was not inhibited by any alcohol, the specificityof phospholipase D was shown not to be affected by double bonds in any alcohol molecule.
The incorporation of cyclic alcohols into PC was more difficult than that of straight chainalcohols, and cyclic alcohols possessing a secondary hydroxyl group were not incorporated atall. That no inositol was incorporated into PC with phospholipase D may thus possible have beendue to inositol's possessing a cyclic structure and no primary hydroxyl group.
