Abstract
Optimal conditions for the quantitative hydrolysis of phosphatidylcholine (PC) into 1, 2-diacylglycerol (DG) were determined using little characterized phospholipase C from Bacillus sp., The hydrolysis ratio in a two-phase medium with solvent was higher than in aqueous medium. The hydrolysis of PC was accelerated in the presence of buffer consisting of disodiurn hydrogenphosphate-potassium dihydrogenphosphate at pH 7.0 and diethyl ether. The optimal conditions for hydrolysis were found to be the following ; PC-90 : 50 mg, diethyl ether : 1 mL, phosphate buffer (pH 7.0) : 6.0 mL, and phospholipase C : 0.02 Unit. PC was hydrolyzed completely within 80 min to form DG under these conditions.
