Abstract
The elution properties of various polar and apolar nonionic compounds on supercritical fluid chromatography (SFC), using a silica gel column were studied. Relationships among capacity factor (k'), adsorption energy (S0), adsorption cross area (As) and solvent strength (ε0), were reported in thin layer chromatography as theoretically [log k' =α (S0-As×ε0) +C] (C, column constant and α, activation factor of the silica gel column). The elution time of each compound could be predicted and chromatographic conditions could be easily established by the proper choice and concentration of modifier. These findings were applied, in a step gradient mode, to the fractionation of toiletry and cosmetic ingredient mixtures. Hydrocarbons, fatty alcohols, alkylglycerylethers, fatty acid mono- and di-ethanolamides were found to be fractionated quite efficiently.
