Abstract
Lipase unit is defined as that amount of enzyme needed to produce 1 μmol non-esterified fatty acid in one minute under specified conditions. Non-esterified fatty acid is traditionally measured by acid-base titration. This was done in the present study in place of High-performance liquid chromatography (HPLC) method. HPLC with a light scattering detector was conducted to measure non-esterified fatty acids to determine the activity of lipase from Candida cylindracea. HPLC result was essentially the same as that obtained by titration, the former method being more sensitive.
